FastQCFastQC Report
Sat 29 Sep 2018
A2_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA2_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31818589
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGAT212200.017.4399221
GCCGAAT130950.016.8573191
GCGGGAT146800.016.3640481
GCCGGGT154750.016.3624761
GGGGGAT202600.015.0972891
GGGGTAT139500.014.9967221
CGGGGGG152900.014.8147631
GGCGGAT131500.014.5924591
CGGGGAT343400.014.0119351
GGCGGGT159600.012.7464361
GCCGGAT98050.012.5811711
GGCGAAT67600.012.3789521
CGGTTAC86150.012.3711543
CCGGGAT166800.012.06648351
GCCGGTT128250.012.0372961
CGGGTAT40200.012.0232331
CGGGAAT91300.011.9310521
GCGGGTT113650.011.8063611
GCGGGGT204100.011.5932021
GTGGATC343500.011.3206892
Produced by FastQC (version 0.11.5)